1459904656-8fc8d6ec-36a8-4b1f-9eb4-e7777b100188

A first recommended route is computed as having the minimum cost from a start point to an end point as a destination. Aspects of the invention include the use of a reduced affinity enzyme complementation reporter system. A DCDC converter assembly is coupled to the PFC assembly, and receives the first DC output and generates a second regulated DC output.