In a method for the intraoperative generating of an updated volume dataset, a first volume dataset is reconstructed from a series of n 2D X-ray projections of the patient are acquired from different angles. In an exemplary embodiment, the remote, self-contained communications antenna unit includes a transportation vehicle that carries a generator for power, backup batteries, transmitter and receiver equipment, a digital signal processor, a network interface unit, an air conditioner, and an extendible mast. The invention provides a compound represented by the following formula. The static instructions or the dynamic instructions are selected for execution at runtime on the first execution unit or the second execution unit, respectively, based on the throughput performance of the instructions. The invention comprises using the library in a variety of selection protocols for identifying, isolating and characterizing known or unknown target RNAs, to reveal the phenotypic effects of such cleavage, and to identify the gene products that produce those phenotypic effects.