The invention disclosed in this patent document relates to transmembrane receptors, particularly to a human G protein-coupled receptor, more particularly to a CXC chemokine receptor subtype 3, and most particularly to mutated version of the human CXCR3 for evidence of constitutive activity and uses thereof. The thin film transistor comprises a first gate electrode and a second electrode being in contact with the first gate electrode and a gate insulating film. Preferably two distinct sets of flow channels exist in which the process proceeds in one set of channels and heat transfer fluid passes through the second. The surfaces and intersection present a self-draining configuration which inhibits the entrapment therein of any deleterious materials that would support the growth of bacteria, fungi, mold and other microorganisms. The substituted instructions result in strength reduction in the computer program. The brake ECU releases braking force when predetermined held-brake release conditions, including accelerator opening degree conditions, for releasing a held brake are satisfied.