This invention concerns novel methods and compositions useful for directly and rapidly analyzing the biochemical components of microorganisms. The process occurs under very mild reaction conditions and with short reaction times. Both lengths of tape are then sealed to the outer bonding layer of the insert. Each side edge has an arcuate upper section having a curvature of a first radius, which terminates at the lower surface of the base. The process of this invention provides for the preparation of HFC-32 by a process that exhibits both good product yield and selectivity. The similarity between any two musical items is then determined as either a function of the distance between the two corresponding vectors.