Disclosed are methods of purifying compounds that reduce or prevent an inflammatory response in a mammal, use of such compounds in treating a mammal having or being at risk of developing inflammation, as well as serum containing such purified compounds. The process works well with clay soils and the range of suitable spoil can be extended by adding cement to the powder material. In some embodiments, the column temperature andor inlet pressure are controlled by a programmable chromatographic oven. The external portion may also prevent insertion of the circuit breaker into a breaker box such that a vent in the circuit breaker housing is blocked. The metal layers stacked in the stacked metal layer are formed with small areas. A body-voltage sense circuit short pulse reading circuit is described using body connected load transistors and a novel sensing circuit with second stage amplifier which allows for very short read pulses providing much higher read margins, less sensing time, and shorter sensing current pulses.