A method of operating an engine is provided. The features provided include rotational therapy, percussion therapy and pulsation therapy on a critical care bed frame with a low air loss patient support, all of which are controlled with various types of feedback from particularized sensors in the bed. The mediumnucleoprotein bound complex may be optionally stored or transported, and is subsequently treated with eluents to remove undesirable proteins and inorganic salts. The sheet is supplied and discharged passing between heater-punches placed above pressuring holes and heater-punches placed under pressuring holes. The surface tension of the, liquid provides a latching mechanism for the relay.