A method for performance tuning a database is described. In particular, the present invention provides an authentication system that operates in conjunction with a secure server to authenticate users to provide access information as needed for the authenticated users to access the secure server. The relay is open upon applying power to the filter and remains open while the capacitor at least partially charges to cause charging current to pass through the resistor and thereby limit in-rush current. The display also has means for creating a potential difference between the two electrodes. The reinforcing member has a plurality of structural pieces separated by slits extending from an inner circumferential surface to an outer circumferential surface of the reinforcing member. Crystallizing IGF-1 comprises the steps of mixing an aqueous solution comprising IGF-1 with a reservoir solution comprising a precipitant to form a mixture; and crystallizing the mixture, optionally also recrystallizing and isolating the crystalline IGF-1.