The present invention relates to an animal chew toy and a method of providing an animal chew toy wherein the toy has a body portion and a core portion. The joint coupling including a body having a bore, with the bore receiving the shaft, and the body including a secondary fastener hole extending normal to the bore. The optical property of the dye is observed after the mixture is exposed to a stimulating means. A ligature surrounds the mouthpiece and reed to secure the reed to the mouthpiece. The casual storage field can receive an information element from another application program, display the received information element in the display region of the casual storage field, and hand over in response to a user instruction, the information element to yet another application program. In addition, this invention concerns a process for producing such an HBV vector as well as cells usable for this purpose.