A display apparatus and a brightness adjusting method thereof are provided. This new assay can detect HCV infection earlier than the currently used assays for the screening of blood for HCV infection by using a combination of HCV antigens and anti-core antibodies to capture HCV. A substrate isolation layer of the PNP bipolar transistor, a drain buried layer of the NMOS transistor, and a back gate buried layer of the PMOS transistor are formed simultaneously by selectively implanting N-type impurities, such as phosphorous, in the semiconductor substrate. Dimples are formed on the outer surface of the golf ball product, including the flared area. , apoptosis, necrosis, cholestasis, andor steatosis.