A novel chemical genus of furan-2-one antifouling compound as non-toxic, environment friendly antifouling agent, a coating material for objects submerged under the water based on the furan-2-one compounds. The undercar assembly has a structural member extending longitudinally between the longitudinal sides of the railroad car, where this structural member includes a pair of longitudinally extending beam elements transversely spaced apart from one another. The serum transfer cup has a higher elevation in the 13 mm sample tube than in the 16 mm sample tube, and the different flange heights can be detected using ultrasonic signals.