Methods of obtaining a single cell expression profile from a target mammalian cell are provided. Exercises engage users in the task of first seeing a grid with angled \u201cbumpers\u201d placed in various places throughout the grid. A filter unit reduces a signal with a specific frequency from the control signal output from the control unit, and passes a remaining signal, and a driving unit drives the axial magnetic bearing based on the signal output from the filter unit. Using a switching means connected to the coils, a magnetic force is generated that causes the rotation of the output shaft. The improved cultivar further is characterized by a mature seed content of less than 20 pounds per 1000 pounds of fruit and by fruit that is readily de-stemmed by mechanized de-stemming. The active devices are configured to operate upon a transient charge flow as a current when in operation.