This invention discloses a substantially protein-free cell culture solution for assisted reproductive technologies and methods of use thereof. Also disclosed are methods for computing IQ impairments from a sampled complex signal, methods for computing DC properties of a signal path between the transmitter and receiver, and methods for transforming IQ impairments through a linear system. The light guiding member has an upper face including a light emission face for emitting light and a lower face including a reflective face, which are arranged in parallel. Within the temperature range of about 900-1600 F. These compounds are useful for the treatment of diseases such as cancer in humans or animals. The method also includes printing the inner surface of at least one of the substrate sheets with a thickness-compensation layer, outside the substrate area intended for receiving the antenna.