Methods and systems for profiling the locations of users of wireless communication terminals. Detection of the reference level and selection of the equalization curve may be performed at a different rates to avoid interfering with one another. A selected gate voltage is applied during the operation to establish the target threshold voltage. An intermediate lens or separation member lies between the first and second shades. These kits circumvent the need for training personnel in plating, growing and count viable cells, and reduce both the time and the cost required for cellular quantitation according to existing techniques. In some embodiments, the quantitative analysis of the ion signal comprises comparing the ion signal intensity with a predefined threshold.