Methods are provided for making a treatment composition by loading active components into a high pressure vessel and pressurizing the high pressure vessel with carbon dioxide to reach a pressure within the high pressure vessel of about 400 pounds per square inch to about 1,070 pounds per square inch. Materials of the present invention may include emissive, photostable organic molecules that are appropriately functionalized to interact with a cell with essentially no disruption in normal cell functioning. The connection assurance device is configured to ensure proper positioning of the clamping yoke on the shaft to allow for final assembly.