A method of forming at least one Micro-Electro-Mechanical System cavity includes forming a first sacrificial cavity layer over a lower wiring layer. Methods of altering quinolate phosphoribosyl transferase expression are provided. To prevent catching or snagging of the tube on the spigot preventing insertion, after forward motion of the spigot, the jaws are opened, and the spigot is retreated a short distance, the jaws are closed and the spigot is advanced again.