The present invention pertains to a method for treating a pulmonary disease state in mammals by altering indigenous in vivo levels of nitric oxide in mammalian cells. Trackable markings are recorded via the camera array, the markings are extracted from the recordings, and a location of the markings is monitored, wherein the spatial position of the object is based on the location of the markings. The bucket size is an interaction area determined by the wavelength of the laser light, the focusing distance, and the 1e2 radius of the near field intensity.