Apparatus is provided that distracts and maintains the distracted position of adjacent vertebral bodies during a surgical procedure within a vertebral disc space. The assay also includes a running buffer that includes a dye or other detectable label that nonspecifically binds only to double stranded nucleic acids. The oil phase and an aqueous phase are present at an OA ratio of from about 1:8 to about 10:1. In another embodiment, the connector includes a translator board having electrical contacts on both sides and electrical circuits within to expand the footprint of the fine pitch electrical device to the coarser pitch of the substrate.