In one aspect, the present invention features a system including a local computing device in communication with a distributed computing system, the local computing device configured to perform computations for a first portion of a computer software application and to send a second portion of the application for computation on the distributed computing system. The present invention is based on the cloning and heterologous expression of genes encoding the chelocardin biosynthetic pathway. The wall has a portion at a distal end thereof, the portion curving inwardly toward an axis of the lumen. The flash removal system replaces the flash-exposed frames with the replacement frames, generating a modified video having reduced flash lighting.