An environment detection device for detecting a plurality of environmental conditions comprising a housing unit. In order to strengthen the heat-sealed joints of the plastic sheets, the plastic sheets are thickened at portions where the heat-sealed joints are to be formed. The present invention further contemplates assays for detecting such viral variants, which assays are useful in monitoring anti-viral therapeutic regimens and in developing new or modified vaccines directed against viral agents and in particular HBV variants. In embodiments of this invention, the synchronization between the blind TCP peers is achieved using a system that consists of a registration server, an agent application, and a virtual network interface that together relay and replicate the control signals between the two TCP peers.