Polyethyleneimine and polyalkylene biguanide ligand functionalized substrates, methods of making ligand functionalized substrates, and methods of using functionalized substrates are disclosed. A predetermined amount of minutes is associated with the set of cards. At least one of the plurality of cam follower members has a protrusion portion opposed to the cylindrical surface, and the interval between the cylindrical surface and the protrusion portion is smaller than that of the bottom surface of the cam groove portion and the end surface of the engagement portion.