Various embodiments of the present invention provide methods for designing multilayer nanowire crossbars that are functionally equivalent to two-layer nanowire-crossbar designs. The method includes the step of providing a tube and the step of coating the tube with polyurethane resin in order to provide a cushion on the tube. The different types of electrophysiological data are combined to provide a graphical depiction that indicates the desired region for the treatment instrument. The temperature of each compartment is individually controlled by a digital temperature controller and a thermoelectric cooling assembly which includes a fan and a cold plate engaged on a thermally conductive heat transfer block at the top of each compartment which engages the bag support housing.