A lycopene cyclase from spinach is described, as is production of lutein in microorganisms by expression of the lycopene cyclase. The metallic holding body is formed with a socket having an inner periphery formed with an annular groove. Also provided are compositions useful to produce the pluripotent embryonic stem cells and methods of screening associated with the method of making the embryonic stem cell. Also disclosed are methods for incorporating or utilizing the disclosed circuits. The connecting part is formed so as to have contact with at least part of the side surface of the first impurity-diffused region.