A method and apparatus for extruding unvulcanized rubber in a tire production process, in which an unvulcanized rubber extruded member can be formed by extrusion. The polynucleotide may be contained in an appropriate expression vector, such as a viral vector. The slurry is then subjected to separation of the sand and bitumen. The upper substrate includes a second transparent substrate. The method may be utilized for patients taking anti-depressants such as tricyclic anti-depressants, monamine oxidase inhibitors or serotonin selective reuptake inhibitors.