A method and apparatus for Flow Injection Analysis into Atmospheric Pressure Ion sources including Electrospray and Atmospheric Pressure Chemical Ionization sources whereby the sampling and spray needles are one and the same. The active growth factor is preferably a composition containing at least one bone morphogenetic protein and a suitable carrier. The enclosure comprises a wall structure extending substantially an entire height of the enclosure. A fuel slinger, affixed to the shaft, receives fuel from an airfuel annulus defined by a stator and a rotor of the generator. The supplemental torque versus engine speed curve intercepts the baseline torque versus engine speed curve at a lower speed point and a higher speed point.