A method is provided that includes generating coefficients of a scene adaptive filter based on differences between values of neighboring pixels in a representative two dimensional image, and applying the SAF to a plurality of corresponding 2D images. The biomolecules are attached directly to the surface of the substrate together with biomolecule-repellent molecules, which cover the surface between the biomolecules to prevent nonspecific binding of analytes and other biomolecules. The present apparatus addresses the new refinements and innovations leading to a self-serve hand-held nozzle system that has an improved overall performance, increased mechanical durability, reduced cost of manufacture, and an easy to use, ergonomic design, allowing for increased comfort and simplistic operation. Afterwards, the iso space pattern constituted by the etched second material layer is formed after the first patterned photoresist layer and the patterned material layer are removed.