A method and apparatus for manipulating a surgical guidewire. This antibody neutralizes infection of the virus in a dose dependent manner. A method of making an electrical pulse generator includes providing one or more piezoelectric elements, a driver, a receiver, and an explosive material and operably associating the explosive material with an outer surface of the driver. The self supporting substrate can be obtained by a method comprising the steps of forming an AlN crystal layer on a single crystal substrate such as a sapphire by the HVPE method, preheating the obtained substrate having the AlN crystal layer to a temperature of 1,200\xb0 C.