An in-mold primer coating adapted to be applied in-mold to a thermoplastic substrate and heat cured to form a thermoset surface coating on the molded substrate. Pump modules may be constructed using two reflector or three reflector VCSEL devices optionally integrated with microlens arrays and other optical components, to couple high power pump beams to an optical fiber output port, particularly suited to couple light to an inner cladding of a double-clad fiber suitable for a high power gain element. A second unit of circuitry is separated from the first unit of circuitry by a free space gap. The system then uses a payload address from the TSO-send message to access a payload from memory that contains message data using remote direct memory access. The method further comprises removing all or a portion of the second sidewall spacer after implanting the sourcedrain region, where the remaining sidewall spacer is narrower following the sourcedrain implant to improve sourcedrain contact resistance and PMD gap fill, and to facilitate inducing stress in the transistor channel. The assay chambers may further be associated with an analysis site for analyzing a sample solution contained within the assay chamber.