Disclosed herein are methods for the isolation and purification of antibodies wherein the use of an affinity chromatographic step results in an antibody composition sufficiently pure for pharmaceutical uses. Sol-form microparticle components contained in CMP wastewater are removed by a filtration treatment using a filter device 13, having a gel-form second filter. The modulation pulse period and repetition rate are adjusted to permit deposition at low aspect ratio and very little or no deposition at high aspect ratio during the deposition cycle and to permit etching of the material deposited on the low aspect ratio area and etching of the material in the high aspect ratio area during the etching cycle. The support structure is preferably sized to support one or two vehicle guideways. Pulsed electrical stimulation to the vagus nerve may be provided continuously in ON-OFF repeating cycles.