A circuit design apparatus of the present invention includes a first element which divides a circuit into a plurality of divided circuits and a second element which calculates individual constraint values of the divided circuits, respectively, using a constraint value of the circuit. The elimination of mannosylphosphorylated glycoproteins results from the disruption of the PNO1 gene and the newly isolated P. Provided is an Ag alloy film useful as a reflecting film andor a transmitting film or as an electrical wiring andor an electrode, including 0.