A force scanning probe microscope and associated method of making force measurements on a sample includes a piezoelectric scanner having a surface that supports the sample so as to move the sample in three orthogonal directions. The substituent can be attached at the 2-position of the functionalized nucleoside via a linking group. A nut is rotatably mounted at a bottom portion of the washer. The bracket is coupled to a transverse frame member of the tonneau cover. A pressure sensor includes a spring having first and second coiled sections offset by a coiled center section in a middle that is used to make offset contact between two electrical contact pads. The cup-holding device has a basket in a seat mounting portion wherein there is sufficient rigidity of the structure to hold a cup with a fluid mass therein.