A rotary cutting tool and method for cutting a shape in a workpiece. A cold accumulating material is sealed within the cold accumulator to be solidified while being cooled by the cold air from the evaporator. The methods of making the compositions including combining the protein, water, acid and optionally ester to form a dispersion without the benefit of alcohol. The stabilizer may be aluminum-based. The support structure can be disposed between the elastomeric membrane and the sample inlet and can provide mechanical support to the elastomeric membrane.