The present application relates to a variant Fc region comprising at least one modification relative to a wild-type human Fc region, where the modification selected from the group consisting of 434S, 252Y428L, 252Y434S, and 428L434S, and the numbering is according to the EU index. The controller is operable to store a fixed code. The tubes are split-flow closed-end tubes inserted from one end of the electrical component. The memory stores a plurality of data files. The method includes depositing a sourcedrain contact layer; depositing a semiconductor layer on the sourcedrain contact layer; and patterning the sourcedrain contact layer and the semiconductor layer substantially simultaneously, wherein the patterned semiconductor layer forms part of a sensor of the sensor array.