A vehicle simulation system has an image system for projecting a simulated out-the-window scene and symbology as produced by a head-up display onto a viewing screen. The auxiliary gateway, on a smart energy network, extracts the unique identifier from a communication related to the smart energy device. The pressure in the interior of the container may be increased by displacing the closure member into the container or by displacing some other element such as a bung into the container. More detailed and other embodiments of the present invention are disclosed in the specification hereof. The method includes contacting the luciferase with a tolerance enhancement agent in an amount sufficient to substantially protect luciferase enzyme activity from interference of the compound and minimize interference by at least about 10% relative to an assay not having tolerance enhancement agent. An active area mask is used to form an active silicon area, and is then reused to form a trench transfer area.