The present invention defines a transaction management contract between an application server and a resource adapter and its underlying resource manager that allows an application server to utilize local transactions on a resource manager and avoid the overhead of an external transaction manager. The invention further relates to vectors and methods for expression and production of enzymatically active high mannose lysosomal enzymes using transgenic plant root, particularly carrot cells. The spools are independently rotatable relative to the shell. A deflector deflects the signal from the emitter toward the plate and from the optical unit towards the receiver. The mechanism may involve passing a bias current across the leads of the superconducting loop and a mechanism for measuring a potential change across the leads of the superconducting loop.