This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, mixed with the nucleic acids of interest, and electrically treated. The present invention encompasses methods preventing respiratory syncytial virus infection in a human, comprising administering to said human a prophylactically effective amount of one or more antibodies or fragments thereof that immunospecifically bind to one or more RSV antigens, wherein a certain serum titer of said antibodies or antibody fragments is achieved in said human subject. Powder in the reservoir is caused to be aerosolized by the propellant pulse whereupon aerosolized powder exits the reservoir through an open end of, and elongate slots provided in the sidewall of, an elongate hollow member extending from an outlet in the receptacle through the stored powdered material into a head space in the reservoir. When one holding brake is engaged, the state of motion of the elevator and any slipping of the brake is monitored. Packets received by the WLAN MAC layer are fragmented into packets each of which is no larger than the fragmentation threshold.