A Toolbox and Instrument software design and runtime system which simulates real-world Tools by providing Instruments for the Tools’ configuration, control, and state monitoring before, during, and after runtime. The first N-type well is disposed in the N-type epitaxial layer under the isolation structure and on one side of the gate. The particle analyzer may further comprise a waste liquid isolating chamber, which causes isolation of the internal fluid passageway connecting the inlet and the outlet of the waste liquid isolating chamber by the interior air. For point-wise LMMSE scaling, the energy of each channel tap is estimated. In various embodiments, the surgical stapler includes a flexible driver operably engaged with the cutting member to move the cutting member relative to the anvil and the staple cartridge.