A method is disclosed for high-throughput screening assay sample preparation and testing for the identification of binding between drug targets and library compounds, for use with a calorimetric device measuring the enthalpy of reaction for the binding. This invention also relates to an associated driving assistance device. The gel may prevent a crystalline resin in the core of the toner particles from migrating to the toner surface. Next, a thermal oxide film is formed on the surface of the semiconductor substrate, and then a thin nitride liner is formed on the thermal oxide film.