An apparatus and method of using the same are provided for generating a gradient of particles within a microfluidic device. The ASR may also comprise a communications device configured to receive a transaction request from an RF transaction device, wherein the transaction request comprises transaction biometric sample data, a reload protocol update request, and an RF device identification code. The interactive geographic interface includes a visual representation which may be manipulated including pan, zoom level, content, filtering, map type, elevation, etc. Improved RA uniformity and less head noise are observed. A second contact provides an electrical connection to the top electrode. 02% to about 2% with respect to the high hardness UV curable resin; an optical initiator having a weight percent of about 0.