An atherectomy and thrombectomy system includes a catheter body extending between proximal and distal catheter portions. The fluorescence illumination system includes a trans-illumination component configured to direct excitation light into a first surface of the specimen wherein diffused light emanates from a second surface thereof for receipt through the view port to acquire fluorescence data of the specimen. The method comprises setting a region of interest for an area tracked by a camera device communicatively coupled to the server in response to a receipt of region of interest configuration data. The check valve closing when a pressure inside the second chamber is larger than a pressure inside the first chamber by a predetermined pressure differential.