The invention relates to a novel Universal RT-coupled PCR strategy for the specific detection and accurate quantitation of mRNA. The hand in the fused image is identified. Each test case version is identified in the test case library by date order sequence number. Pins may be coupled between the support structure and the frame to substantially thermally isolate the support structure from the frame. The first reflecting surface is formed in such a manner that a distance L in a vertical direction from the LED to the first reflecting surface is approximately 10 mm in a preferred embodiment. During the execution, the distributed runtime environment identifies isolated runtime constructs included in the software application and selects distributed runtime constructs corresponding to the isolated runtime constructs.