The present invention relates to recombinant DNA encoding the PpuMI restriction endonuclease as well as PpuMI methylase, expression of PpuMI restriction endonuclease and PpuMI methylase in E. In one preferred embodiment, the artificial ligament is in the form of a flexible conformable plate dimensioned to span adjacent vertebrae and having openings for reception of bone screws, fasteners, etc. Therefore, it is possible to the gate insulating film applied electric field of the MIS transistor in the unselected state lower than the electric field in the selected state to assure the reliability of the gate insulating film of the MIS transistor in the unselected state. Such solution both applies to a phase of initial establishment of air interface self-synchronization between base stations, and also to a phase of periodic synchronization maintenance. This permits the completely read postal articles to be immediately subjected to a fine sorting and enables this time to be used for offline video encoding the incompletely read postal articles.