1459902231-4c83edc0-d615-4730-a34c-161dd9c7a81b

The invention provides an energy transfer apparatus having an energy transfer chamber in which rotating flow is established. The combined expression of the replicon RNA and the helper RNA in the nidovirus permissive cell produces an assembled nidovirus particle which comprises the heterologous RNA sequence, is able to infect a cell, and is unable to complete viral replication in the absence of the helper RNA due to the absence of the structural protein coding sequence in the packaged replicon. The bracket includes a sidewall and a bottom wall. With an appropriate width for each waveguide, an arrayed waveguide grating is provided that has low polarization dependent wavelength. The outer jacket can be provided with break-away features allowing the jacket to be peeled off of a pacing lead.