Methods and compositions suitable for accomplishing the in vitro amplification of nucleic acid molecules via enzymatic means are provided. The cartridge comprises: a cartridge frame; a memory unit; a first portion disposed at an upstream portion in a vicinity of an upstream end in an attachment direction of the cartridge frame; and a first terminal disposed at at least a part of the first portion and electrically connected to the memory unit. Each signal conductor has a first contact end connectable to a printed circuit board, a second contact end, and an intermediate portion having a first width. 5 part by weight of sulfur per 100 parts by weight of the rubber base, has a hardness difference between the surface and center thereof, expressed in JIS-C hardness units, of at least 21 but not more than 30, and has a diameter of from 36 to 40 mm. Vigorous pulsing of the fluid exiting the bit nozzles results in better cleaning of the hole bottom and faster drilling. In one embodiment, the polymer contains a hydroxyalkyl acrylate ester which is crosslinked with a titanium-containing chelated metal alkoxide.