A method for calibrating a filter begins with the filter filtering a first signal having a first frequency to produce a first filtered signal, wherein the first frequency is in a known pass region of the filter. According to the invention, the shutoff, anchor and sealing means include hydraulic activation means such that the hydraulic pressure transmitted from the surface activates the means so as to open the passage, anchor the valve in the tubing and activate the sealing means on the wall of the tubing. The ligands chelate to a BF2 center to produce the fluorescent dye. The data is then forwarded from the multiplexer via the SCSI emulator to a PCI bus, the PCI bus being physically connected with the peripheral’s engine. Moldings of these polymers are distinguished by high low-temperature notched impact strength associated with a high modulus of elasticity. The prosthetic implant includes a generally spherical or ellipsoidal body that at least partially engages a surface of adjacent vertebrae.