The invention relates to methods for training players and sportsmen. The device includes a sleeve that defines an opening, and a plunger to which a suture thread can be attached. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. The first, second and third substrate form a multi-layer body structure having at least one edge extending between a first side of the first substrate and the second side of the third substrate. Some of the functions which used to be performed by software are now accelerated through hardware.