A therapeutic agent for treating polycystic ovary syndrome, which exhibits few, if any, side effects, is effective at inducing ovulation, and is safely available for not only females who desire to bear children, but also for unmarried or young females. The vascular closure device includes an improved plug that is configured to be positioned adjacent to the hole in the vasculature. A plurality of angular segments mounted in an annulus formed in proximity to the perimeter of the dies adapted for controlled radial travel of the angular segments during rotation of the dies, each angular segment having at least one ejector pin for radial reciprocal travel in a die recess, ejects punch-out material from the recess. The lyophilisates have a surprisingly long shelf life and are surprisingly stable.