A method of manufacturing an integrated circuit may include the steps of annealing a gate structure and a halo section disposed over a substrate using a first temperature, implanting dopants to form drain and source regions, and annealing drain and source regions at a second temperature. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, \u03b2-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa, and EG V. The invention also provides synthetic procedures for preparing said compounds and certain intermediates, as well as intermediates themselves.