Compositions that include Toll-like Receptor 5 agonists and at least a portion of at least one viral antigen can be employed in methods that stimulate an immune response in a subject, in particular, a protective immune response in a subject. A weighted frame difference computation unit 3423 outputs weighted difference. Calling of the clock control library and changing of the register value are controlled by an application program. The generated routing graph includes nodes and route links that connect the plurality of nodes.