A device and method for conditioning a periodic analog signal to predetermined positive and negative desired peak values. The design guidelines include the application of design rules to some but not all functional blocks of the chip, may be stored on a computer-readable medium and the design of the semiconductor ic chip and the generation of a photomask set for manufacturing the semiconductor ic chip may be carried out using a CAD or other automated design system. The rate of nitrification is a product of the nitrifying bacteria biomass concentration and the specific growth rate of the bacteria. A second transistor has a source electrode and a drain electrode individually overlapping the second electrode portion but not the second end portion EP2) in the pixel region. The motion estimator may utilize an enhanced block matching technique to perform block matching based on stationary and spatially proximate blocks. The separate functions are performed by different structures within the fluid couplings.