Improved installation features for recessed light fixtures including downlights are provided. The apparatus includes a vortex generation zone shaped as an inverse truncated cone, a solids collection zone, a separation zone, one or more fluid inlets, one or more gas inlets, one or more fluid outlets, and one or more gas outlets. A determination is made if the pathloss is less than or larger than a predetermined value. , R2 represents a group represented by formula: etc. The mRNP complex is collected by removing the mRNP complex from the solid support.